detection of isoniazid-resistant clinical isolates of mycobacterium tuberculosis from india using ser315thr marker by comparison of molecular methods
نویسندگان
چکیده
in this study, substitution at codon ser315 of katg gene, a reliable marker for isoniazid (inh) resistance was analyzed and compared by three molecular methods such as dna sequencing, polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) and pcr-single strand conformation polymorphism (pcr-sscp) in 105 phenotypically resistant isolates obtained from various parts of india. out of the 105 resistant isolates, 64 (61%) were found to be resistant by dna sequencing, 54 (51%) by pcr-rflp and 57 (54%) by pcr-sscp methods. the results obtained using pcr-sscp and pcr-rflp methods were compared with those from dna sequencing (gold standard). the sensitivity and specificity of pcr-rflp were 84% and 100% respectively and corresponding values for pcr-sscp method were 89% and 95% respectively. the study has shown the comparison of the simple, rapid and cost effective methods with dna sequencing targeting codon ser315 of katg gene and suggests that pcr-rflp and pcr-sscp may be performed as alternative inexpensive methods in settings with a high prevalence of inh-resistant m. tuberculosis strains where sequencing cannot be afforded.
منابع مشابه
Detection of Isoniazid-Resistant Clinical isolates of Mycobacterium tuberculosis from India using Ser315Thr marker by Comparison of molecular methods
In this study, Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance was analyzed and compared by three molecular methods such as DNA sequencing, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-single strand conformation polymorphism (PCR-SSCP) in 105 phenotypically resistant isolates obtained from various parts of Ind...
متن کاملVirulence in isoniazid-resistant clinical isolates of Mycobacterium tuberculosis from south India
Isoniazid, is the only antituberculous drug for which the relation between lack of virulence and acquisition of resistance was associated. INH-resistant mutants were shown to contain defective katG gene. Classical studies showed that INH-resistant south Indian isolates have lower virulence in guinea pigs and higher susceptibility to H2O2. It is of interest to assess the virulence in south India...
متن کاملvirulence in isoniazid-resistant clinical isolates of mycobacterium tuberculosis from south india
isoniazid, is the only antituberculous drug for which the relation between lack of virulence and acquisition of resistance was associated. inh-resistant mutants were shown to contain defective katg gene. classical studies showed that inh-resistant south indian isolates have lower virulence in guinea pigs and higher susceptibility to h2o2. it is of interest to assess the virulence in south india...
متن کاملVirulence in isoniazid-resistant clinical isolates of Mycobacterium tuberculosis from south India
متن کامل
Molecular characterisation of isoniazid resistant clinical isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan.
OBJECTIVE To investigate the frequency of mutations in catalase-peroxidase and inhibin alpha genes in clinical isolates of isoniazid resistant Mycobacterium tuberculosis strains. METHODS The study was conducted at Provincial Tuberculosis Reference Laboratory, Peshawar, Pakistan, from April 2015 to March 2016, and comprised sputum specimens obtained from patients of different ages. All the iso...
متن کاملDetection of Methicillin-Resistant Staphylococcus Aureus by Phenotypical and Molecular Methods among Clinical Isolates
Abstract Background and Objective: Increasing prevalence of methicillin resistant Staphylococcus aureus strains (MRSA) with their multidrug resistance potential causes difficulties in the treatment of infections due to these bacteria. Hence, the detection and determination of the frequency of MRSA strains via phenotypical and molecular methods is necessary in different parts of the county. ...
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عنوان ژورنال:
international journal of molecular and clinical microbiologyجلد ۱، شماره ۲، صفحات ۵۲-۵۹
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